THLE-3 細胞，人肝永生化細胞

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細胞原代牛、狗、雞、鴨、魚原代細胞羊原代細胞豬原代細胞兔原代細胞小鼠原代細胞大鼠原代細胞人原代細胞培養(yǎng)基原代細胞凍存液 ATCC 人乳腺癌豬正常細胞豬睪丸細胞系人膠質(zhì)瘤細胞人成骨細胞人永生化肝細胞人肝癌細胞人卵巢癌細胞人結(jié)直腸腺癌人經(jīng)典小細胞肺癌細胞人小細胞肺癌細胞人結(jié)直腸腺癌細胞人鼻腔上皮細胞中國倉鼠卵巢懸浮細胞嗜性逆轉(zhuǎn)錄病毒包裝穩(wěn)定細胞株小鼠神經(jīng)細胞瘤人眼脈洛膜黑色素瘤 PT67 逆轉(zhuǎn)錄酶病毒包裝細胞鴨子胚胎成纖維細胞豬腎細胞系昆蟲細胞貓腎細胞恒河猴胚腎細胞倉鼠卵巢細胞負鼠腎細胞倉鼠肺細胞果蠅胚胎細胞家貓肺成纖維樣細胞雞胚細胞豚鼠心肌來源細胞豚鼠肺成纖維樣細胞豚鼠皮膚成纖維樣細胞狗腎細胞非洲綠猴胚胎腎上皮細胞猴胚胎腎上皮細胞長臂猿淋巴瘤新生牛眼囊成纖維細胞黑化大家鼠肺成纖維樣細胞俄勒岡地鼠細胞家兔皮膚成纖維樣細胞昆蟲卵巢細胞人胸膜瘤細胞非洲綠猴腎細胞 VERO 衍生株（SVP）白化金黃地鼠肺成纖維樣細胞黃胸鼠肺成纖維樣細胞白鰱尾鰭細胞蝙蝠肺細胞草魚腎細胞系大黃魚腎細胞猴腎細胞系犬腎細胞系人結(jié)腸直腸腺癌豬肺泡巨噬細胞 PFHSA05（TLL2）人重組特洛德樣蛋白因子 PFHSA04 （BMP1）人重組骨誘導因子人乳腺癌細胞結(jié)腸癌細胞成纖維細胞人胚腎細胞動物卵巢細胞動物胚胎細胞動物肺細胞肋骨來源細胞成纖維樣細胞動物上皮細胞動物肝臟細胞可誘導表達穩(wěn)定細胞株動物氣管細胞動物腎細胞動物巨噬細胞人正常細胞人腫瘤細胞、癌細胞小鼠正常細胞小鼠腫瘤細胞、癌細胞大鼠正常細胞大鼠腫瘤細胞其它動物細胞查看全部產(chǎn)品

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THLE-3 細胞，人肝永生化細胞

traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 0.1% Soybean Trypsin inhibitor and aspirate cells by gently pipetting.

5.To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 x g for 5 to 10 minutes.

6.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new coated culture vessels.

7.Place culture vessels in incubators at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

THLE-3 細胞，人肝永生化細胞

Flask Coating

1.Prepare a mixture of 0.01 mg/mL fibronectin, 0.03 mg/mL bovine collagen type I and 0.01 mg/mL bovine serum albumin (BSA) dissolved in culture medium. Store pre-prepared Coating Solution at 4°C in cold room for up to 3 months.

2.For a growth area of 75 cm2, add 4.5 mL of the fibronectin/collagen/BSA solution and rock gently to coat the entire surface.

3.Incubate the freshly coated vessel(s) in a 37°C incubator overnight (it is preferable to use tissue culture vessels with tightened, plug-seal caps to prevent evaporation during the coating process).

4.Store coated flasks with solution at room temperature, light protected, up to 1 month. Suction off solution before plating cells.

Cryopreservation Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions Temperature: 37°C

STR Profile Amelogenin: X

CSF1PO: 11,12

D13S317: 13

D16S539: 11,12

D5S818: 13

D7S820: 8,10

THO1: 8,9.3

TPOX: 6,9

vWA: 17,18

Name of Depositor National Cancer Institute

References Harris CC, et al. Human liver epithelial cells. US Patent 5,759,765 dated Jun 2 1998

Pfeifer AM, et al. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. USA 90: 5123-5127, 1993. PubMed: 7685115

References Harris CC, et al. Human liver epithelial cells. US Patent 5,759,765 dated Jun 2 1998

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