国产精品A免费一区久久电影_天干天干天啪啪夜爽爽99_chinesevideo国产熟妇_新金瓶悔1一5集免费大片

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠腫瘤細(xì)胞、癌細(xì)胞>CRL-1838M-NFS-60 小鼠白血病細(xì)胞G-CSF依賴性

M-NFS-60 小鼠白血病細(xì)胞G-CSF依賴性

簡要描述:CRL-1838 M-NFS-60 小鼠白血病細(xì)胞G-CSF依賴性,
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,

  • 產(chǎn)品型號(hào):CRL-1838
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:2280

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

CRL-1838 M-NFS-60 小鼠白血病細(xì)胞G-CSF依賴性, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件,

CRL-1838 M-NFS-60 小鼠白血病細(xì)胞G-CSF依賴性 的詳細(xì)介紹

CRL-1838

ATCC® Number:CRL-1838™  Price:$349.00
Designations:M-NFS-60

Depositors:JF Weaver, A Creasey

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:suspension

Organism:Mus musculus (mouse)

Morphology:lymphoblast


Source:Disease: myelogenous leukemia
Cell Type: virus induced


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Applications:transfection host (Roche FuGENE® Transfection Reagents)

Comments:The M-NFS-60 cell line was derived from a myelogenous leukemia induced with the Cas-Br-MuLV wild mouse ecotropic retrovirus.

The cells are responsive to both interleukin 3 (interleukin-3, IL-3) and macrophage colony stimulating factor (M-CSF).

The cells contain a truncated c-myb proto - oncogene caused by integration of a retrovirus.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • 0.05 mM 2-mercaptoethanol

  • 62 ng/ml human recombinant macrophage colony stimulating factor (M-CSF)

  • fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0°C



Subculturing:Medium Renewal: Add fresh medium at the time of subculture

Cultures can be maintained by addition or replacement of fresh medium. Subcuture every two days at 2.5 X 10 exp4 viable cells/ml.



Preservation:culture medium 95%; DMSO, 5%

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020



References:22842: Nakoinz I, et al. Differentiation of the IL-3-dependent NFS-60 cell line and adaption to growth in macrophage colony-stimulating factor. J. Immunol. 145: 860-864, 1990. PubMed: 2142710

23295: Weinstein Y, et al. Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line. Proc. Natl. Acad. Sci. USA 83: 5010-5014, 1986. PubMed: 3014527



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸